When you acquire images either from a digital camera or from a film scanner, the images are not calibrated for one-to-one (1:1), life-size output. In contrast, if you are scanning images using a flatbed scanner, there is no depth of field involved and you specify the size of the area to be scanned, so images acquired using this particular digital device are acquired as true, life-size images.
Unless, of course, you are scanning an image that has been enlarged, such as a latent fingerprint that was photographed and then printed as an 8 by 10 inch photograph, which may or may not be to a particular scale. Upon scanning this photograph, it would be necessary to rescale the image to print a life-size image or to send the image to an automated fingerprint identification system (AFIS).
But returning to our original scenario, let’s say you are using a Kodak DCS 330 digital camera. The CCD chip has a resolution of 2008 pixels by 1504 pixels. You can photograph a one-inch by one-inch area or you can photograph a three-by-five-inch area; it makes absolutely no difference to the camera. The camera has no idea how large the area is that it just captured. All it knows is that you captured an area that consists of 2008 pixels by 1504 pixels.
Using Photoshop, you can accurately calibrate any image as long as the image has a scale in it. The technique works by counting the number of pixels from one known point on the scale to another known point on the scale, thus determining the number of pixels per inch for resolution.
NOTE: There are three elements that make up image size of any digital image. First there are the “measurable” physical attributes: width and height. Then there is a more obscure dimension known as resolution (AKA pixels per inch). By knowing one of the measurable attributes, you can determine the other two attributes of image size.
So how do you calibrate your images? Using Photoshop, it is a simple, two-step process. First you crop the image, then you determine what this distance is, and Photoshop does the rest. Here are the actual steps to perform this process.
Figure 1. Image window showing placement of the marching ants
NOTE: For accurate scaling, ensure that the marching ants go directly through the center of the scale bar because the width of the scale bar can vary depending upon the resolution of the CCD, the size of the area captured, and so forth. For example, an individual scale bar can be 13 pixels wide or it can be 23 pixels wide. Therefore, by counting the exact number of pixels from the center of one scale bar to the center of another scale bar gives you a more accurate pixel count.
Figure 2. Enlargement of a single scale marker;
the width of this
scale bar is 13 pixels.
Figure 3. Image Menu
Figure 4. Image Size dialog box shows the link of width,
height,
and resolution fields
NOTE: We recommend that you use a metric scale to make it easier for determining the numeric value to be entered into the width or height field. For example, ¼ inch, is easy, you simply enter 0.25 for the known distance. If you have 1/8th of an inch, the size is 0.125; 1/16th of an inch is 0.0625, and so forth. If you have 3/16th of an inch, it is 0.1875. (OK, in Ski’s world you have officially entered “math hell!” With a metric scale, all you have to do is count the number of scale markers and divide by 10. Hence 24 mm equals 2.4 cm; 13 mm equals 1.3 cm, and so forth. It is much easier)
Figure 5. Image Size dialog box showing the unit of
measurement
changed for width, and the correct distance entered in the
width field